mb mannan Search Results


recombinant human mannose binding lectin  (Bio-Techne corporation)
90
Bio-Techne corporation recombinant human mannose binding lectin
Glycan epitopes in D. immitis . a A summary of neutral and anionic N-glycan structures highlighting major antennal modifications; for a fuller set of defined structures refer to Supplementary Table . b The presence of HexNAc and phosphorylcholine residues in L3 larvae was probed using CGL3 (red) and TEPC 15 (green) by indirect fluorescence microscopy; also shown are the DAPI staining (for DNA) and DIC (differential interference contrast) images and the scale bar corresponds to 10 µm. c MALDI-TOF–MS of the AEAB-labelled neutral N-glycome before and after hydrofluoric acid treatment indicating loss of antennal fucose and phosphorylcholine residues. d The binding of Coprinopsis galectin (CGL3; 10 µg/ml), wheat germ agglutinin (WGA; 10 µg/ml) and human mannose binding <t>lectin</t> (MBL) to the Dirofilaria glycans increases after hydrofluoric acid treatment, while that to anti-phosphorylcholine (TEPC 15 IgA monoclonal) decreases. The charts indicate the uncorrected fluorescence values with the standard deviations (mean of 10 spots; analysed with an unpaired two-tailed parametric t -test with a 95% confidence level) as well as a negative control (spotting buffer only; example array scans are shown for the WGA and MBL data). Note that previous data indicate that CGL3 can recognise LacdiNAc, whereas WGA is known to bind HexNAc n motifs and MBL to a wide range of glycans including those with terminal mannose or N -acetylglucosamine residues. Refer to Supplementary Figures – and for (i) western blotting and further micrograph data (including controls) on epitopes recognised by CGL3 and TEPC 15, (ii) a summary of MS data on proteins affinity purified on CGL3- and TEPC 15-Sepharose, (iii) further MBL binding experiments (before and after endoglycosidase H treatment) to the immobilised glycome pools and fractionated immobilised glycans as well as data on other lectin interactions to the natural glycans and to defined di- and tri-saccharides
Recombinant Human Mannose Binding Lectin, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mb+mannan/Recombinant+Human+MBL+Protein/pmc06325117-249-51-56
Average 90 stars, based on 1 article reviews
recombinant human mannose binding lectin - by Bioz Stars, 2026-10
90/100 stars
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mb mannan  (R&D Systems)
91
R&D Systems mb mannan
Glycan epitopes in D. immitis . a A summary of neutral and anionic N-glycan structures highlighting major antennal modifications; for a fuller set of defined structures refer to Supplementary Table . b The presence of HexNAc and phosphorylcholine residues in L3 larvae was probed using CGL3 (red) and TEPC 15 (green) by indirect fluorescence microscopy; also shown are the DAPI staining (for DNA) and DIC (differential interference contrast) images and the scale bar corresponds to 10 µm. c MALDI-TOF–MS of the AEAB-labelled neutral N-glycome before and after hydrofluoric acid treatment indicating loss of antennal fucose and phosphorylcholine residues. d The binding of Coprinopsis galectin (CGL3; 10 µg/ml), wheat germ agglutinin (WGA; 10 µg/ml) and human mannose binding <t>lectin</t> (MBL) to the Dirofilaria glycans increases after hydrofluoric acid treatment, while that to anti-phosphorylcholine (TEPC 15 IgA monoclonal) decreases. The charts indicate the uncorrected fluorescence values with the standard deviations (mean of 10 spots; analysed with an unpaired two-tailed parametric t -test with a 95% confidence level) as well as a negative control (spotting buffer only; example array scans are shown for the WGA and MBL data). Note that previous data indicate that CGL3 can recognise LacdiNAc, whereas WGA is known to bind HexNAc n motifs and MBL to a wide range of glycans including those with terminal mannose or N -acetylglucosamine residues. Refer to Supplementary Figures – and for (i) western blotting and further micrograph data (including controls) on epitopes recognised by CGL3 and TEPC 15, (ii) a summary of MS data on proteins affinity purified on CGL3- and TEPC 15-Sepharose, (iii) further MBL binding experiments (before and after endoglycosidase H treatment) to the immobilised glycome pools and fractionated immobilised glycans as well as data on other lectin interactions to the natural glycans and to defined di- and tri-saccharides
Mb Mannan, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mb+mannan/Recombinant+Human+MBL+Protein%2C+CF/pm37689061-687-12-10
Average 91 stars, based on 1 article reviews
mb mannan - by Bioz Stars, 2026-10
91/100 stars
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mannan binding lectin mbl2 mouse monoclonal antibody  (OriGene)
N/A
MBL2 mouse monoclonal antibody clone OTI2G5 formerly 2G5
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mbl oligomer elisa kit  (Fitzgerald Industries International)
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ELISA Kit for the determination of oligomerized Mannan binding lectin MBL in human serum or heparin plasma
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mannan binding lectin mbl2 nm 000242 human recombinant protein  (OriGene)
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Recombinant protein of human mannose binding lectin protein C 2 soluble opsonic defect MBL2
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anti-mbl2 (internal region) polyclonal antibody  (Creative BioMart)
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Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic
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goat anti-human mbl2 / mannan-binding lectin, (c terminus)  (RayBiotech)
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Goat Anti-Human MBL2 / Mannan-Binding Lectin, (C Terminus)
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recombinant human complement masp3/mannan-binding lectin serine protease 1/masp1  (Beijing Solarbio Science)
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human mannan binding lectin / mbl2 gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Human mannose binding lectin 2.
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rat mannan binding lectin / mbl2 gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
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Full length Clone DNA of Rattus norvegicus mannose-binding lectin (protein C) 2.
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umbelopsis ramanniana (möller) gams  (ATCC)
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Image Search Results


Glycan epitopes in D. immitis . a A summary of neutral and anionic N-glycan structures highlighting major antennal modifications; for a fuller set of defined structures refer to Supplementary Table . b The presence of HexNAc and phosphorylcholine residues in L3 larvae was probed using CGL3 (red) and TEPC 15 (green) by indirect fluorescence microscopy; also shown are the DAPI staining (for DNA) and DIC (differential interference contrast) images and the scale bar corresponds to 10 µm. c MALDI-TOF–MS of the AEAB-labelled neutral N-glycome before and after hydrofluoric acid treatment indicating loss of antennal fucose and phosphorylcholine residues. d The binding of Coprinopsis galectin (CGL3; 10 µg/ml), wheat germ agglutinin (WGA; 10 µg/ml) and human mannose binding lectin (MBL) to the Dirofilaria glycans increases after hydrofluoric acid treatment, while that to anti-phosphorylcholine (TEPC 15 IgA monoclonal) decreases. The charts indicate the uncorrected fluorescence values with the standard deviations (mean of 10 spots; analysed with an unpaired two-tailed parametric t -test with a 95% confidence level) as well as a negative control (spotting buffer only; example array scans are shown for the WGA and MBL data). Note that previous data indicate that CGL3 can recognise LacdiNAc, whereas WGA is known to bind HexNAc n motifs and MBL to a wide range of glycans including those with terminal mannose or N -acetylglucosamine residues. Refer to Supplementary Figures – and for (i) western blotting and further micrograph data (including controls) on epitopes recognised by CGL3 and TEPC 15, (ii) a summary of MS data on proteins affinity purified on CGL3- and TEPC 15-Sepharose, (iii) further MBL binding experiments (before and after endoglycosidase H treatment) to the immobilised glycome pools and fractionated immobilised glycans as well as data on other lectin interactions to the natural glycans and to defined di- and tri-saccharides

Journal: Nature Communications

Article Title: Highly modified and immunoactive N-glycans of the canine heartworm

doi: 10.1038/s41467-018-07948-7

Figure Lengend Snippet: Glycan epitopes in D. immitis . a A summary of neutral and anionic N-glycan structures highlighting major antennal modifications; for a fuller set of defined structures refer to Supplementary Table . b The presence of HexNAc and phosphorylcholine residues in L3 larvae was probed using CGL3 (red) and TEPC 15 (green) by indirect fluorescence microscopy; also shown are the DAPI staining (for DNA) and DIC (differential interference contrast) images and the scale bar corresponds to 10 µm. c MALDI-TOF–MS of the AEAB-labelled neutral N-glycome before and after hydrofluoric acid treatment indicating loss of antennal fucose and phosphorylcholine residues. d The binding of Coprinopsis galectin (CGL3; 10 µg/ml), wheat germ agglutinin (WGA; 10 µg/ml) and human mannose binding lectin (MBL) to the Dirofilaria glycans increases after hydrofluoric acid treatment, while that to anti-phosphorylcholine (TEPC 15 IgA monoclonal) decreases. The charts indicate the uncorrected fluorescence values with the standard deviations (mean of 10 spots; analysed with an unpaired two-tailed parametric t -test with a 95% confidence level) as well as a negative control (spotting buffer only; example array scans are shown for the WGA and MBL data). Note that previous data indicate that CGL3 can recognise LacdiNAc, whereas WGA is known to bind HexNAc n motifs and MBL to a wide range of glycans including those with terminal mannose or N -acetylglucosamine residues. Refer to Supplementary Figures – and for (i) western blotting and further micrograph data (including controls) on epitopes recognised by CGL3 and TEPC 15, (ii) a summary of MS data on proteins affinity purified on CGL3- and TEPC 15-Sepharose, (iii) further MBL binding experiments (before and after endoglycosidase H treatment) to the immobilised glycome pools and fractionated immobilised glycans as well as data on other lectin interactions to the natural glycans and to defined di- and tri-saccharides

Article Snippet: The slides were incubated with either dilutions of dog sera (one control and two infected), biotinylated fungal CGL3 and CCL2 (see above) or biotinylated forms of wheat germ agglutinin, Aleuria aurantia lectin, concanavalin A or tomato lectin (Vector Laboratories; B1025, B1395, B1005 and B1175), murine TEPC 15 IgA monoclonal (Sigma-Aldrich; M1421), recombinant human mannose binding lectin (Biotechne; 9085-MB-050) or natural human C-reactive protein (MPBio; 215231505) followed by the relevant secondary and/or tertiary antibodies.

Techniques: Fluorescence, Microscopy, Staining, Binding Assay, Two Tailed Test, Negative Control, Western Blot, Affinity Purification